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Bio-Microbiology Lab - School of Applied STEM - Universitas Prasetiya Mulya

Bio-Microbiology Lab

ABOUT THE LABORATORY

This lab is used to study microbes and their application to food, either to test quality based on the presence of microbial contaminants or to improve quality by microbial fermentation. The laboratory is equipped with Biosafety Level II which is suitable for experiments involving agents with moderate hazard potential for personnel and the environment

Equipment Usability

  • PCR (Polymerase Chain Reaction) RT PCR is used for real-time and end-point thermal cycling using polymerase chain reaction (PCR) and high-resolution melting analysis (HMR) for various molecular biology applications.
  • Biosafety CabinetBiosafety Cabinet (BSC) is a instrument used to work aseptically. BSC has an air filter setting thus become steril and application of UV light a few hours before use. This instrument protect personnel against biohazardous or infectious agents and help to maintain quality control of the material being worked with as it filters both the inflow and exhaust air.
  • Colony CounterColony counters are used to estimate a liquid culture’s density of microorganisms by counting individual colonies on an agar plate, slide, mini gel, or Petri dish. This instruments accommodate various sizes and formats, including units that can scan plates up to 300 × 300 mm, and are optimized for use with UV illumination, white light, fluorescent, and/or Green Fluorescent Protein colonies. The counting can be accomplished manually, often with touch pressure and a digital counter, or can be semi- or fully automatic. Typical applications include Ames testing, bacterial mutation assays, and E. coli bacterial colonies.
  • Incubator & ShakerThe incubator shaker is an instrument controlled by temperature combining incubation and shaking functions. This instrument is used for cell cultrure, fermentation, hybridization, biochemistry and research of enzyme and cell tissues which require a high quality of temperature control and shaking speed. The incubator shaker able to incorporate oxygen and evenly distribute nutrients throughout the culture media.
  • Analytical BalanceAnalytical balances are precision measuring instruments used in quantitative chemical analysis, to determine the mass of solid objects, liquids, powders and granular substances. Their readability has a range between 0.1mg - 0.01mg. Analytical balances have a draft shield or weighing chamber to prevent the very small samples from being affected by air currents.
  • Microscope BinocularBinocular microscope is a type of light microscope with two eyepieces to significantly ease viewing and cut down on eye strain and one objective. This instrument is used to magnify a small object or to magnify the fine details of a larger object in order to examine minute specimens that cannot be seen by the naked eye.
  • Microscope TrinocularTrinocular microscope is a binocular microscope equipped with a lens for photographic recording during direct visual observation. This microscope allows for the user to not only take pictures, but also record videos which can be saved for future references.
  • MicropipetteMicropipettes are used to accurately and precisely deliver small volumes of liquid. This device measure a much smaller volume, starting at 1 microliter and usually extensively used in molecular diagnostics.
  • Autoclave for sterilizationAn autoclave that uses steam under pressure to kill harmful bacteria, viruses, fungi, and spores on items that are placed inside a pressure vessel
  • Autoclave for destructionAn autoclave that is uses to sterilise waste and destroy microorganism. Operating in a similar fashion to a traditional pressure cooker.
  • E-loopThe electrical sterilization system E-Loop is used for sterilization of inoculation loops, needles and instruments. The sterilizing tube, which is made of wear-resistant quartz glass, reaches its optimal temperature of 900°C (1650°F) after only a few minutes. To sterilize the inoculation loop, simply insert it into the quartz tube. After 5-7 seconds, the inoculation loop is sterilized.
  • MicrowaveAn oven which cooks food very quickly by electromagnetic radiation rather than by heat. This device is used to heat Microbiology medium
  • Microsmash Bead Cell DisruptorAn oven which cooks food very quickly by electromagnetic radiation rather than by heat. This device is used to heat Microbiology medium
  • NanoPhotometer UV-Vis SpectrophotometerThe Implen NanoPhotometer™ N50 UV/VIS spectrophotometer is the right instrument when it comes to the quantification of nucleic acids, proteins or cell cultures as it offers scan data from 200 - 650 nm in just 0.3 - 2 µL of sample.
  • Thermo ShakerTS-100C are intended for extensive mixing of samples in microtest tubes or PCR plates in a temperature-controlled environment. This device is capable of cooling samples to +4°C.

Courses Using Bio-Microbiology Lab

  • Final Project
  • Basic Biology
  • Microbiology
  • Food Microbiology